Short Answer Type

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'DNA replication is semi-conservative'. Explain.


Replication of DNA involves the unwinding of the double-helix structure. Each single strand then acts as a template for the synthesis of a new DNA molecule again with a specific base pairing. During this replication, only half of the parental DNA is conserved and only one strand is synthesised. That’s why DNA replication is semiconservative.
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What are forces holds the two strands of DNA together in a double-helix structure? Draw structure to show the bonding between adenine and thymine, and between guanine and cytosine.

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Long Answer Type

What happens when D-glucose is treated with following reagents?
(i) Acetic anhydride                  
(ii) Hydroxylamine
(iii) Tollen’s reagents              
(iv) Fehling solution
(v) Phenyl hydrazine.
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Short Answer Type

Explain the following:
Glucose responds Fehling solution and osazone, it does not react with Schiff’s base and sodium bisulphite.

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Which vitamin is called ascorbic acid? What are its physiological functions? Which disease is caused by the deficiency of this vitamin?

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Explain the following:
Although fructose does not have an aldehydic group, it reacts with Fehling solution.

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What is melting temperature (Tm) of DNA? A DNA molecule with more number of GC base pairs than AT base pairs has higher Tm than the one with lesser number of GC base pairs than AT base pairs. Explain why?

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Explain the following:
Glucosides react neither with Fehling solution nor with Tollen's reagent. They also do not allow mutarotation.

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What do you understand by a vitaminoses? Name the defeiciency disease caused due to lack of Vitamin A and K.
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What is transcription with reference to protein synthesis? How does it take place?

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