Multiple Choice Questions

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The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of 

  • Non-recombinant bacteria containing  β-galactosidease 

  • Insertional inactivation of α - galactosidase in recombinant bacteria

  • Insertional inactivation of α-galactosidase in recombinant bacteria

  • Insertional inactivation of α-galactosidase in recombinant bacteria


C.

Insertional inactivation of α-galactosidase in recombinant bacteria

The colonies of recombinant bacteria appear white in contrast to blue calories of non-recombinant bacteria because of insertional inactivation of alpha-galactosidase in recombinant bacteria. Alpha-galactosidase is a glycoside hydrolase enzyme that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoprotein. It in encoded by theGLA gene. β-galactosidase is an exoglycosidase, which hydrolyzes the β-glycosidic bond formed between a galactose and its organic rhoiety.

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Which of the following is not a feature of the plasmids?

  • Circular structure

  • Transferable

  • Single stranded

  • Single stranded

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DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by

  • centrifugation

  • polymerase chain reaction

  • electrophoresis

  • electrophoresis

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Which of the following is a restriction endonuclease?

  • Protease

  • DNase I

  • RNase

  • RNase

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The DNA molecule to which the gene of interest is integrated for cloning is called

  • Transformer

  • Vector

  • Template

  • Template

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The introduction of t-DNA into plates involves

  • Infection of the plant by agrobacterium tumefaciens

  • Altering the pH of the soil, then heat-shocking the plants

  • Exposing the plates to cold for a brief period

  • Exposing the plates to cold for a brief period

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The figure below is the diagrammatic representation o the E.Coli vector pBR 322. Which one of the given options correctly identifies its certain components(s)?

  • Ori- original restriction enzyme

  • rop-reduced osmotic pressure

  • Hind III, Eco RI-selectable markers

  • Hind III, Eco RI-selectable markers

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The cutting of DNA at specific locations become possible with the discovery of

  • Restriction enzymes

  • Probes

  • Selectable markers

  • Selectable markers

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The Taq polymerase enzyme is obtained from

  • Thiobacillus ferroxidans

  • Bacillus subtilis

  • Pseudomonas subtilis

  • Pseudomonas subtilis

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Which of the following is not correctly matched for the organism and its cell wall degrading enzyme?

  • Bacteria -Lysozyme

  • Plant cells - Cellulase

  • Algae - Methylase

  • Algae - Methylase

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