Multiple Choice Questions

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Cry-I endotoxins obtained from Bacillus thringiensis are effective against 

  • mosquitoes

  • flies

  • nematodes

  • nematodes


B.

flies

Insect-resistant transgenic plants contain either a gene from the bacterium Bacillus thuringiensis or some other gene. The cry gene of bacillus thuringiensis produces a protein which forms crystalline inclusion in the bacterial strains. Cry-I endotoxins are effective gains Lepidoptera (eg flies). Cry- V and Cry-VI proteins are effective against are ingested by insects, they are dissolved in the alkaline juices present in the midgut lumen. The gut proteases process them hydrolytically to release the core toxic fragments. The toxic fragments are believed to bind to specific high-affinity receptors present in the brush border of midgut epithelial cells and causing their swelling and eventual lysis.

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What is antissense technology? 

  • A cell displaying a foreign antigen used for synthesis of antigens

  • Production of somaclonal variants in tissue cultures. 

  • When a piece of RNA that is complementary to sequence is used to stop expression of a specific gene.

  • When a piece of RNA that is complementary to sequence is used to stop expression of a specific gene.

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Which one of the following pairs of nitrogenous bases of nucleic acids, is wrongly matched with the category mentioned against it?

  • Thymine, Uracil - Pyrimidines

  • Uracil, Cytosine - Pyrimidines

  • Guanine, Adenine - Purines

  • Guanine, Adenine - Purines

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During transcription, RNA polymerase holoenzyme binds to a gene promoter and assumes a saddle-like structure, what is it’s DNA-binding sequence?

  • TTAA

  • AATT

  • CACC

  • CACC

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The two polynucleotide chains in DNA are

  • parallel

  • discontinuous

  • antiparallel

  • antiparallel

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Telomere repetitive DNA sequences control the function of eukaryotic chromosomes because they

  • act as replicons.

  • are RNA transcription initiator

  • help chromosome pairing

  • help chromosome pairing

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Differentiation of organs and tissues in a developing organism, is associated with

  • developmental mutations

  • differential expression of genes

  • lethal mutations

  • lethal mutations

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The Okazaki fragments in DNA chain growth

  • result in transcription

  • polymerize in the 3’ - to - 5’ direction and forms replication fork

  • prove semi-conservative natuire of DNA replication

  • prove semi-conservative natuire of DNA replication

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Molecular basis of organ differentiation dpends on the modulation in transcription by

  • RNA polymerase

  • ribosome

  • transcription factor

  • transcription factor

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The linking of antibiotic resistance gene with the plasmid vector became possible with

  • DNA ligase

  • endonucleases

  • DNA polymerase

  • DNA polymerase

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