Multiple Choice Questions

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What is antissense technology? 

  • A cell displaying a foreign antigen used for synthesis of antigens

  • Production of somaclonal variants in tissue cultures. 

  • When a piece of RNA that is complementary to sequence is used to stop expression of a specific gene.

  • When a piece of RNA that is complementary to sequence is used to stop expression of a specific gene.


C.

When a piece of RNA that is complementary to sequence is used to stop expression of a specific gene.

The antisense RNA technology simply involves the synthesis of RNA molecules that are complementary to the mRNA molecules produced by transcription of a given gene.  Usually t his antisense RNA will not contain the regulatory sequences required for translation but even if does it will not usually specify a functional product. Instead, most antisense RNAs contain multiple termination codons in all three reading frames. However, when antisense RNA molecules are present in the same cytosol with sense (mRNA) molecules of a gene, the antisense RNA and mRNA molecules will anneal to form duplex RNA molecules. These duplex RNA molecules cannot be translated thus, the presence of antisense RNA will block translation of the mRNA of the affected gene.

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The linking of antibiotic resistance gene with the plasmid vector became possible with

  • DNA ligase

  • endonucleases

  • DNA polymerase

  • DNA polymerase

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Telomere repetitive DNA sequences control the function of eukaryotic chromosomes because they

  • act as replicons.

  • are RNA transcription initiator

  • help chromosome pairing

  • help chromosome pairing

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The two polynucleotide chains in DNA are

  • parallel

  • discontinuous

  • antiparallel

  • antiparallel

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The Okazaki fragments in DNA chain growth

  • result in transcription

  • polymerize in the 3’ - to - 5’ direction and forms replication fork

  • prove semi-conservative natuire of DNA replication

  • prove semi-conservative natuire of DNA replication

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Molecular basis of organ differentiation dpends on the modulation in transcription by

  • RNA polymerase

  • ribosome

  • transcription factor

  • transcription factor

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Which one of the following pairs of nitrogenous bases of nucleic acids, is wrongly matched with the category mentioned against it?

  • Thymine, Uracil - Pyrimidines

  • Uracil, Cytosine - Pyrimidines

  • Guanine, Adenine - Purines

  • Guanine, Adenine - Purines

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During transcription, RNA polymerase holoenzyme binds to a gene promoter and assumes a saddle-like structure, what is it’s DNA-binding sequence?

  • TTAA

  • AATT

  • CACC

  • CACC

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Differentiation of organs and tissues in a developing organism, is associated with

  • developmental mutations

  • differential expression of genes

  • lethal mutations

  • lethal mutations

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Cry-I endotoxins obtained from Bacillus thringiensis are effective against 

  • mosquitoes

  • flies

  • nematodes

  • nematodes

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