The linking of antibiotic resistance gene with the plasmid vector became possible with
DNA ligase
endonucleases
DNA polymerase
DNA polymerase
A.
DNA ligase
DNA ligase is used to seal the nicks that remain in recombinant DNA molecule. In fact, DNA ligase joins together the neighbouring nucleotides flanking a discontinuity in a DNA strand by forming a phosphodiester bond. A recombinant DNA molecules is a vector into which the desired DNA fragment has been inserted to enable its cloning in an appropriate host.
Endonucleases are the enzymes that produce internal cuts called cleavage in DNA molecules. A class of endonucleases cleaves DNA only within or near those sites, which has specific base sequences, such endonucleases are known as restriction endonucleases.
Exonucleases are enzymes that remove one or more nucleotide from the free ends.
DNA polymerase is the enzyme, which causes polymerization of nucleotides during DNA replication.
What is antissense technology?
A cell displaying a foreign antigen used for synthesis of antigens
Production of somaclonal variants in tissue cultures.
When a piece of RNA that is complementary to sequence is used to stop expression of a specific gene.
When a piece of RNA that is complementary to sequence is used to stop expression of a specific gene.
The Okazaki fragments in DNA chain growth
result in transcription
polymerize in the 3’ - to - 5’ direction and forms replication fork
prove semi-conservative natuire of DNA replication
prove semi-conservative natuire of DNA replication
During transcription, RNA polymerase holoenzyme binds to a gene promoter and assumes a saddle-like structure, what is it’s DNA-binding sequence?
TTAA
AATT
CACC
CACC
Telomere repetitive DNA sequences control the function of eukaryotic chromosomes because they
act as replicons.
are RNA transcription initiator
help chromosome pairing
help chromosome pairing
Differentiation of organs and tissues in a developing organism, is associated with
developmental mutations
differential expression of genes
lethal mutations
lethal mutations
Molecular basis of organ differentiation dpends on the modulation in transcription by
RNA polymerase
ribosome
transcription factor
transcription factor
Cry-I endotoxins obtained from Bacillus thringiensis are effective against
mosquitoes
flies
nematodes
nematodes
Which one of the following pairs of nitrogenous bases of nucleic acids, is wrongly matched with the category mentioned against it?
Thymine, Uracil - Pyrimidines
Uracil, Cytosine - Pyrimidines
Guanine, Adenine - Purines
Guanine, Adenine - Purines
