Short Answer TypeWhat is the effect of pH on the activity of enzymes?
Enzymes are highly sensitive to a change of pH value. Since enzymes are proteins, pH change greatly affect the ionic character of amino and carboxylic acid groups on the protein surface. This change affects the catalytic nature of an enzyme also. The enzymes function within a narrow range of pH value. For example,
Enzyme Pepsin Ptyalin Trypsin pH range 1.8 to 2.2 5.6. to 6.6 7.5. to 8.3
The low or high pH value can cause considerable denaturation of the protein and hence makes enzyme protein inactive.
The two samples of DNA, A and B have melting temperatures 340 and 350K respectively. Draw conclusion from this data regarding their base content.
How will you show the presence of,
(i) primary alcoholic group.
(ii) Six carbon atoms in a straight chain in glucose.
A tipeptide on complete hydrolysis give glycine, alanine and phenyl alanine. Using three letter symbols, write down the possible sequences of the tripeptide.
If two amino acids: alanine and phenyl alanine react together, how many possible dipeptides can be formed? Write down the structures and names of each one. Also, write their names using three and one letter abbreviation for each amino acids.
Which bonds in the backbone of a peptide can rotate freely and which cannot? Give reasons.
Name the purine and pyrimidine bases present in DNA and RNA. Give the structure of any one of the pyrimidine base.
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