Protoplast fusion is used in plant tissue culture for various applications.

In protoplast fusion:

(A) naked plant cells are used.

(B) transfer of organelles is not possible.

(C) partial genome transfer is involved.

(D) cells from two different plants can be mixed together and forced to fuse.

Which one of the following combinations of the above statements is correct?

• (A), (B) and (C)

• (A), (C) and (D)

• (A), (B) and (D)

• (B), (C) and (D)

Which of the following is a mismatch between the plant drug and its source?

• Codeine - Papaver somniferum

• Vineblastine - Catharanthus roseus

• Quinine - Cinchona ledgeriana

• Digitalin - Artemisia annua

Which of the following curves correctly represents the process of ethanol production yeast?

34.

Inbreeding for 5 generations led to the production of homozygous transgenic mice. However, these homozygous males or females were infertile. Which of the following approach is most preferable and economical to obtain heterozygous transgenic animals continuously?

• Moretransgenic founder (1^st animal) should be generated.

• Crossing (breeding) of transgenic mice with wild type mice in earlier generations should be done for the continued production of transgenic heterozygous offspring.

• Inbreeding should be avoided after 5^th generation.

• Homozygous transgenic mice should be mated with wild type for continued production of transgenic heterozygous offspring.

Which of the following is the most appropriate spectral bands for vegetation analysis using remote sensing platforms?

• Red, Near-infrared

• Infrared, Visible

• Red, Microwave

• Visible, Microwave

Which of the following microbial fermentations are anaerobic?

• Ethanol and acetone-butanol

• Citric acid and propionic acid

• Penicillin and Vitamin B₁₂

• Streptomycin and rifampicin

Encasing of which of the following plant cells in a gelatinous matrix is referred to as artificial seed?

• Microcalli

• Somatic embryos

• Root tips

• Shoot tips

Which of the following transgenic crop(s) have been approved for commercial cultivation in India?

• Cotton

• Brinjal

• Cotton and brinjal

• Cotton, Brasica, Brinjal

Electrons are transferred from reduced co-enzymes like NADH, NADPH to pyruvic acid or its derivatives during fermentation. Thos final electrons acceptors are reduced to the end-products for example lactic acid, propionic acid, etc. The end products depend on the particular microorganism and the substrate

Organism	End product
A. Streptococcus	a. Butanediol, formic acid
B. Clostridium	b. Lactic acid
C. Salmonella	c. Butyric acid, isopropanol
D. Enterobacter	d. Succinic acid, acetic acid

The correct match is

• A - a; B - b; C - c; D - d

• A - d; B - c; C - a; D - c

• A - b; B - c; C - d; D - a

• A - c; B - d; C - a; D - b

A transgenic plant is developed with the following T-DNA construct

In order to analyze the nature of integration, genomic DNA digested with EcoRI was used for Southern hybridization using either probe A or B. The result obtained is as

The following conclusions were made:

A. There are two copies of the T-DNA cassette integrated at one loci and a third copy at another loci.

B. There are two copies of the T-DNA cassette integrated at one loci.

C. Complete T-DNA cassette has been integrated in all cases.

D. In one T-DNA cassette there is a truncation towards the RB.

E. In one T-DNA cassette there is a truncation towards the LB.

F. The arrangement of the T-DNA cassettes integrated at the same loci is

G. The arrangement of the T-DNA cassette integrated at the same loci is

Which of the above are correct?

• B, E and G

• B, D and F

• A, D and F

• B, C and F