Combination of molecular markers with their classification based on either dominant or co-dominant types are shown below:

A. SSR and RFLP: co-dominant

B. SSR and RAPD: co-dominant

C. RAPD and RFLP: dominant

D. AFLP and RAPD: dominant

Which one of the following is the correct combination?

• A and B

• B and C

• C and D

• A and D

Following sets of Plasmodium falciparum sporozoites

(i) normal sporozoites

(ii) sporozoites with mutation in the C-terminal of circumsporozoite (CS) antigen.

(iii) sporozoites with mutation in the N-terminal region of circumsporozoite (CS) antigen

are injected into 2 groups of mice, one normal (Group A) and one (Group B) where localized knockdown of heparan sulfate (receptor for CS antigen in liver cells) is achieved by injecting specific shRNA expressing lentiviral particles in the liver prior to infection. 15 days post-infection, parasitemia is measured by counting infected RBC through Giemsa staining.

Which of the following groups will show maximum level of parasitemia?

• Group B with set (i)

• Group A with set (ii)

• Group A with set (iii)

• Group B with set (ii)

Four groups of one-day female BALB/c neonates had received the following treatments:

A. Epidermal cells from C57BL/6 male plus anti-β2 microglobulin antibody.

B. Epidermal cells from C57BL/6 male plus antibodies, to β2 microglobulin, CD40 ligand, CD80, and CD86.

C. Epidermal cells from C57BL/6 female plus anti-CD80 antibody.

D. Epidermal cells from C57BL/6 female mice.

When these BALB/c neonates grew six weeks old, they received skin transplants from C57BL/6 male mice. Transplantation rejection time varied between these four groups. Starting from the fastest to the slowest rejection, which one of the following is the most likely order?

• D ≥ C > A > B

• A > B > C ≥ D

• B > A > D ≥ C

• D > B ≥ C > A

Cultured animal cells were transfected with expression vector encoding either β-galactosidase (β) alone or expressing a fusion protein of -gal and glucocorticoid receptor (GR). After transfection, cells were kept in presence or absence of Dexamethasone. Immunofluorescence with a labeled antibody specific for β-gal was used to detect the expressed protein in cytoplasm or nucleus of tranfected cells. Possible results of the experiments are:

A. Expression of β-gal alone in the cytoplasm in both absence or presence of Dexamethasone.

B. Expression of β-gal-GR in the cytoplasm in the absence of Dexamethasone.

C. Expression of β-gal alone in the nucleus both in the presence or absence of Dexamethasone.

D. Expression of β-gal-GR in the nucleus in presence of Dexamethasone.

E. Expression of β-dal alone in both cytoplasm and nucleus in presence or absence of Dexamethasone.

F. Expression of v-gal-GR in both cytoplasm and nucleus in presence of Dexamethasone.

Choose the correct combination of results from the following options.

• B, C and D

• A, B and D

• B, D and E

• A, B and F

The number of seeds in the fruit of a plant species, H₀ : μ=30. A random sample of 9 fruits gives the mean number of seeds as 24 with a standard deviation of 6.12. (a) What are the confidence limits for the sample mean? (b) Would you reject or accept the null-hypothesis at 95% confidence level?

• (a) 18 and 30; (b) reject the hypothesis

• (a) 20 and 28; (b) reject the hypothesis

• (a) 20 and 28; (b) accept the hypothesis

• (a) 18 and 30; (b) accept the hypothesis

In an attempt to detect protein expression profile in a cell, Western blot technique is employed. Expression of two new proteins is to be followed by probing with respective high affinity antibodies (raised in rabbit). Unfortunately, the two proteins were found to co-migrate in SDS-PAGE profile. Under this situation, using one-dimensional SDS-PAGE and by Western blot, which one of the following is the best way to demonstrate the presence of both the proteins?

• Develop Western blots with their antibodies in the same gel.

• Prior to doing SDS-PAGE/ Western blot, one protein could be removed by immune precipitating in the cell extracts.

• Silencing the expression of one protein at a time by siRNA and performing Western blotting.

• Subjecting the technique of stripping/ reprobing of the gel after transferring to nitrocellulose membrane while doing Western blotting.

A researcher was repeating a FACS experiment but somehow got confused with the labeling of the tubes. There are four tubes, one control, C (with no fluorescent label), one standard 1, S1 (with FITC label), one standard 2, S2 (with PE label) and the last one test, T (which should be FITC) positive. Given below is the result of the FACS experiment.

What should be the correct labeling?

• a,S2; b,S1; c,T; d,C

• a,S1; b,T; c,C; d, S2

• a,S2; b,S1/T; c,C; d,S1/T

• a,S1/T; b,S2; c,S1/T; d,C

A student was asked to design a knockout cassette for specifically deleting the p53 gene from the prostate gland of mice. Which one of the following pairs of cassettes will ensure the deletion of the gene?

239.

You have isolated a short chain polypeptide from a bacteria which upon administering to animal cells in culture is demonstrated to get transported to mitochondria and to cause mitochondrial membrane disruption. This peptide is being considered for its therapeutic effects on cancer and is to be tested for its anti-cancer properties in the following models of the disease.

A. Cancer with Apaf-1 mutation

B. Cancer where Bax genes are inactivated.

C. Cancer where Bak genes are inactivated.

Which of the following is likely to provide best effect (i.e. induce apoptosis)

• Only A

• B and C

• A and B

• A and C

A promoter deletion study was done in order to determine the binding sites for a transcription factor on the promoter, which is activated on treatment with the drug 'X'. The following constructs were made-

Luciferase assay revealed the following results:

The following statements can be made.

A. Region between -1800 and -1210 contains a binding site for the activator.

B. Region between -868 and -1210 contains a binding site for a repressor.

C. Region between -868 and -432 contains a binding site for a repressor. 

D. Region between -1210 and -868 contains a binding site for the activator.

Which of the above is/are true?

• A and C

• B and C

• A and D

• B only