Which of the following is a restriction endonuclease?

• Protease

• DNase I

• RNase

• RNase

Which of the following is not a feature of the plasmids?

• Circular structure

• Transferable

• Single stranded

• Single stranded

The Taq polymerase enzyme is obtained from

• Thiobacillus ferroxidans

• Bacillus subtilis

• Pseudomonas subtilis

• Pseudomonas subtilis

DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by

• centrifugation

• polymerase chain reaction

• electrophoresis

• electrophoresis

Which of the following is not correctly matched for the organism and its cell wall degrading enzyme?

• Bacteria -Lysozyme

• Plant cells - Cellulase

• Algae - Methylase

• Algae - Methylase

The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of 

• Non-recombinant bacteria containing  β-galactosidease 

• Insertional inactivation of α - galactosidase in recombinant bacteria

• Insertional inactivation of α-galactosidase in recombinant bacteria

• Insertional inactivation of α-galactosidase in recombinant bacteria

The cutting of DNA at specific locations become possible with the discovery of

• Restriction enzymes

• Probes

• Selectable markers

• Selectable markers

The introduction of t-DNA into plates involves

• Infection of the plant by agrobacterium tumefaciens

• Altering the pH of the soil, then heat-shocking the plants

• Exposing the plates to cold for a brief period

• Exposing the plates to cold for a brief period

9.

The DNA molecule to which the gene of interest is integrated for cloning is called

• Transformer

• Vector

• Template

• Template

The figure below is the diagrammatic representation o the E.Coli vector pBR 322. Which one of the given options correctly identifies its certain components(s)?

• Ori- original restriction enzyme

• rop-reduced osmotic pressure

• Hind III, Eco RI-selectable markers

• Hind III, Eco RI-selectable markers