The linking of antibiotic resistance gene with the plasmid vecto

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 Multiple Choice QuestionsMultiple Choice Questions

61.

Which one of the following pairs of nitrogenous bases of nucleic acids, is wrongly matched with the category mentioned against it?

  • Thymine, Uracil - Pyrimidines

  • Uracil, Cytosine - Pyrimidines

  • Guanine, Adenine - Purines

  • Guanine, Adenine - Purines

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62.

Cry-I endotoxins obtained from Bacillus thringiensis are effective against 

  • mosquitoes

  • flies

  • nematodes

  • nematodes

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63.

What is antissense technology? 

  • A cell displaying a foreign antigen used for synthesis of antigens

  • Production of somaclonal variants in tissue cultures. 

  • When a piece of RNA that is complementary to sequence is used to stop expression of a specific gene.

  • When a piece of RNA that is complementary to sequence is used to stop expression of a specific gene.

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64.

The linking of antibiotic resistance gene with the plasmid vector became possible with

  • DNA ligase

  • endonucleases

  • DNA polymerase

  • DNA polymerase


A.

DNA ligase

DNA ligase is used to seal the nicks that remain in recombinant DNA molecule. In fact, DNA ligase joins together the neighbouring nucleotides flanking a discontinuity in a DNA strand by forming a phosphodiester bond. A recombinant DNA molecules is a vector into which the desired DNA fragment has been inserted to enable its cloning in an appropriate host. 
Endonucleases are the enzymes that produce internal cuts called cleavage in DNA molecules. A class of endonucleases cleaves DNA only within or near those sites, which has specific base sequences, such endonucleases are known as restriction endonucleases. 
Exonucleases are enzymes that remove one or more nucleotide from the free ends.

DNA polymerase is the enzyme, which causes polymerization of nucleotides during DNA replication.

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65.

Telomere repetitive DNA sequences control the function of eukaryotic chromosomes because they

  • act as replicons.

  • are RNA transcription initiator

  • help chromosome pairing

  • help chromosome pairing

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66.

Molecular basis of organ differentiation dpends on the modulation in transcription by

  • RNA polymerase

  • ribosome

  • transcription factor

  • transcription factor

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67.

During transcription, RNA polymerase holoenzyme binds to a gene promoter and assumes a saddle-like structure, what is it’s DNA-binding sequence?

  • TTAA

  • AATT

  • CACC

  • CACC

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68.

Differentiation of organs and tissues in a developing organism, is associated with

  • developmental mutations

  • differential expression of genes

  • lethal mutations

  • lethal mutations

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69.

The Okazaki fragments in DNA chain growth

  • result in transcription

  • polymerize in the 3’ - to - 5’ direction and forms replication fork

  • prove semi-conservative natuire of DNA replication

  • prove semi-conservative natuire of DNA replication

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70.

The two polynucleotide chains in DNA are

  • parallel

  • discontinuous

  • antiparallel

  • antiparallel

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