For developing transgenic mice, embryonic stem cells are engineered to express the transgene. These cells are selected by
novobiocin
neomycin
tetracycline
penicillin
B.
neomycin
A positive selectable gene marker such as the neomycin resistance is present in a targeting vector. Therefore, for developing transgenic mice, embryonic stem cells are engineered with neomycin to express the transgene.
t1/2 of an irreversible first-order reaction, S → P is 1 hour. the time (in hours) required to reach 75% completion is
1.5
2.0
2.5
3.0
Which of the following atomic nuclei cannot be probed by nuclear magnetic resonance spectroscopy?
1H
31P
18O
15N
One aims to find out the role of a gene product in macrophages by using a transgenic mouse expressing the genes under a promoter. Which of the following is the most appropriate promoter?
Actin promoter
MHC Class II promoter
Mac-1/ CD 11b promoter
IL-2 promoter
The molar absorption coefficient of phenylalanine is 200 M-1cm-1 at257 nm. What concentration (g/L) of this amino acid will give an absorption of 1 in a cell of 0.5 cm path length at 257 nm?
3.30
0.33
1.65
0.17
Evolution of multi-gene family occurs by
only gene duplication
only unequal crossing over
random mutations
both duplication and unequal crossing over
The speciation in which a population splits into two geographically isolated populations experience dissimilar selective pressure and genetic drift is known as
sympatric speciation
parapatric speciation
peripatric speciation
allopatric speciation
In the case of monoclonal antibody production by hybridoma technology, myeloma cells used to lack the enzyme hypoxanthine-guanine phosphoribosyltransferase (HGPRT) such that fused can only survive when selected on hypoxanthine-aminopterin-thymidine (HAT). What is the role of aminopterin in this medium?
To be used as cell cycle inhibitor of myeloma cells
To block the pathway for nucleotide synthesis
To facilitate fusion of myeloid B cells and antibody-producing B cells
To facilitate production of antibody-producing B cells
Microbial leaching involves the process of dissolution of metals from ore breaking rocks using micro-organisms. Which one of the following bacteria helps in leaching copper from its ore?
Acidithiobacillus ferroxidans
Pseudomonas putida
Deinococcus radiodurans
Rhodopseudomonos capsulate
Which of the following genes was engineered in the "Flavr Savr" transgenic tomato variety?
1-Amino cyclopropane-1-carboxylic acid synthase
1-Amino cyclopropane-1-carboxylic acid oxidase
Expansin
Polygalactouronase