For identification of three proteins moving together (as a single band) upon loading in a single lane of a SDS-PAGE gel, the best method is:

• one step Western blot

• NMR spectroscopy

• Western blot followed by stripping and reprobing

• ESR spectroscopy

312.

Which one of the following techniques will you use to identify more than 1000 differentially expressed genes in normal and tumor tissues in one single experiment?

• RAPD

• Genome sequencing

• ChIP assay

• Transcriptome analysis

Neomycin phosphotransferase gene, frequently used as a selection marker during plant transformation, inactivates which one of the following antibiotics?

• Hygromycin

• Ampicillin

• Streptomycin

• Kanamycin

Which among the following is the simplest method to estimate the concentration of glycerol in an aqueous solution of glycerol?

• UV absorption spectroscopy

• Gas chromatography

• pH measurement

• Viscosity measurement

A film projector and microscope give equal magnification. But a film projector is not used to see living cells because

• a living cell cannot be placed in a film projector.

• the viewer's eye is close to a microscope whereas it is far away from the projector's screen.

• a microscope produces a virtual image whereas a projector produces a real image.

• a microscope has greater resolving power than a projector.

Four circles of unit radius each are drawn such that each one touches two others and their centres lie on the vertices of a square. The area of the region enclosed between the circles is

• $\mathrm{\pi }$ - 1

• $\mathrm{\pi }$ - 2

• 3 - $\mathrm{\pi }$

• 4 - $\mathrm{\pi }$

A turtle swimming from a point A located on the circumference of a circular pond. After swimming for 4 meters in a straight line it hits pond. From there it changes direction and swims for 3 meters in a straight line and arrives at point D diametrically opposite to point A. How far is point D from A?

• 3m

• 4 m

• 7 m

• 5 m

Density of a rice grain is 1.5 g/cc and bulk density of rice heap is 0.80 g/cc. If a 1 litre container is completely filled with rice, what will be the approximate volume  of pore space in the container?

• 350 cc

• 465 cc

• 550 cc

• 665 cc

If D + I + M = 1501

C + I + V + I + L = 157

L + I + V + I + D = 557

C + I + V + I + C = 207

What is V + I + M = ?

• Cannot be found

• 1009

• 1006

• 509

A car is moving at 60 km/h. The instantaneous velocity of the upper most points of its wheels is

• 60 km/h forward

• 120 km/h forward

• 60 km/h backward

• 120 km/h backward