Multiple Choice QuestionsWhich one of the following also acts as a catalyst in a bacterial cell?
sn RNA
hn RNA
23 S rRNA
23 S rRNA
Silencing of mRNA has been used in producing transgenic plants resistant to
bollworms
nematodes
White rusts
White rusts
B.
nematodes
RNA interference (RNAi) takes place in all eukaryotic organisms as a method of cellular defence. This method involves silencing of a specific mRNA due to a complementary ds RNA molecule that binds to an prevents translation of the mRNA (silencing). The source of the complementary RNA could be from an infection by viruses having RNA genome of mobile genetic elements (transposons) that replicate via an RNA intermediate. Using Agrobacterium vectors, nematode -specific genes were introduced into the host plant. The introduction of DNA was such t hat it produced both sense and anti -sense RNA in the host cells. These two RNA's being complementary to each other formed a dsRNA that initiated RNAi and thus, silenced the specific mRNA of the nematode. The result was that the parasite could not survive in a transgenic host expressing specific interfering RNA. The transgenic plant, therefore, got itself protected from the parasite.
The unequivocal proof of DNA as the genetic material came from the studies on a
bacterium
fungus
viroid
viroid
Which one of the following techniques made it possible to genetically engineer living organism
Recombinant DNA techniques
X -ray diffraction
Heavier isotope labelling
Heavier isotope labelling
Which one of the following is used as a vector for cloning the gene into higher organisms?
Baculovirus
Salmonella typhimurium
Rhizopus nigricans
Rhizopus nigricans
The one aspect which is not a salient feature of genetic code is its being
degenerate
ambiguous
universal
universal
Which one of the following palindromic base sequences in DNA can be easily cut at about the middle be some particular restriction enzyme?
5'-CGTTCG -3'
3'-ATCCTA-5'
5'-GATATG-3'
3'-CTACTA-5'
5'-GAATTC-3'
3'CTTAAG-5'
5'-GAATTC-3'
3'CTTAAG-5'
DNA and RNA segment tagged wit a radioactive molecule is called
vector
probe
clone
clone
Genetic engineering has been successfully used for producing
transgenic mice for testing safety to polio vaccine before use in humans
transgenic models for studying new treatments for certain cardiac diseases
transgenic cow -Rosie which produces high-fat milk for making ghee
transgenic cow -Rosie which produces high-fat milk for making ghee
Satellite DNA is useful tool in
Organ transplantation
sex determination
forensic science
forensic science
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