Which one of the following makes use of RNA as a template to synthesize DNA?
Reverse transcriptase
DNA dependant RNA polymerase
DNA polymerase
RNA polymerase
During transcription holoenzyme RNA polymerase binds to a DNA sequence and the DNA assumes a saddle like structure at that point. What is that sequence called?
CAAT box
GGTT box
AAAT box
TATA box
Which one of the following hydrolysis internal phosphodiester bonds in a polynucleotide chain?
Lipase
Exonuclease
Endonuclease
Protease
E. coli cells with a mutated Z gene of the lac operon cannot grow in medium containing only lactose as the source of energy because
in the presence of glucose, E. coli cells do not utilize lactose
they cannot transport lactose from the medium into the cell
the lac operon is constitutevely active in these cells
they cannot synthesize functional - galactosidase
In split genes the coding sequences are called
introns
exons
cistrons
operons
B.
exons
Split genes were discovered by Phillip Sharp and Robert Richard. The part of DNA which express itself by making mRNA is called exon. One exon is separated from others by inactive part of DNA called intron. This is called split DNA.
DNA replication generally proceeds in a
5 3 direction
3 5 direction
3 3 direction
5 5 direction
DNA polymerase enzyme is required for synthesis of
DNA from RNA
RNA from DNA
DNA from DNA
RNA from RNA