Which one of the following makes use of RNA as a template to synthesize DNA?
Reverse transcriptase
DNA dependant RNA polymerase
DNA polymerase
RNA polymerase
During transcription holoenzyme RNA polymerase binds to a DNA sequence and the DNA assumes a saddle like structure at that point. What is that sequence called?
CAAT box
GGTT box
AAAT box
TATA box
Which one of the following hydrolysis internal phosphodiester bonds in a polynucleotide chain?
Lipase
Exonuclease
Endonuclease
Protease
E. coli cells with a mutated Z gene of the lac operon cannot grow in medium containing only lactose as the source of energy because
in the presence of glucose, E. coli cells do not utilize lactose
they cannot transport lactose from the medium into the cell
the lac operon is constitutevely active in these cells
they cannot synthesize functional - galactosidase
DNA replication generally proceeds in a
5 3 direction
3 5 direction
3 3 direction
5 5 direction
A.
5 3 direction
DNA is made up of double helix of two complementary strands. Both the strands get seperated during replication. Each strand of the original DNA molecule then serves as a template for the production of its counterpart, hence, a process is known as semi- conservative replication.
DNA synthesis or replication always proceeds in 5' 3' direction because DNA polymerase acts on the 3'- OH of the existing strand for adding the free nucleotides.
DNA polymerase enzyme is required for synthesis of
DNA from RNA
RNA from DNA
DNA from DNA
RNA from RNA