CBSE
Multiple Choice QuestionsPaul berg's gene splicing experiment created the first rDNA molecule, which was a
T4 phage fragment incorporated into SV40 vector
Lambda phage fragment incorporated into SV40 vector
T4 phage fragment incorporated into pSC 101 vector
Lambda phage fragment incorporated into pSC 101 vector
Viral genome incorporated into host DNA is called
prophase
plasmid
prophage
bacteriophage
The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of
Non-recombinant bacteria containing -galactosidase
lnsertional inactivation of -galactosidase in non-recombinant bacteria
lnsertional inactivation of -galactosidase in recombinant bacteria
Inactivation of glycosidase enzyme in recombinant bacteria
DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by
centrifugation
polymerase chain reaction
electrophoresis
restriction mapping
The sequence given below 5'-GGCC-3' is recognition sequence for which of the following enzyme
Not 1
Eco R1
Hae III
Puv I
Recognition sequence in Eco RI is
GGCC
GAATTC
AAGCTT
CTGCAG
The structure of E. coli chromosomal DNA is
double- stranded, right handed and circular
single- stranded, right handed and circular
double- stranded, left handed and linea
double- stranded, left handed and circular
Passage cells help in
transport of water towards pericycle
transport of water towards epiblema
absorption of water from soil
passage of CO2 towards stomata
The usual source of restriction endonucleases used in gene cloning is from
fungi
bacteria
plants
viruses
During gene cloning, the enzyme used to join the insert DNA with the plasmid vector is
DNA ligase
restriction endonuclease
alkaline phosphatase
exonuclease
