State what is apomixis. Comment on its significance. How can it be commercially used?
During a monohybrid cross involving a tall pea plant with a dwarf pea plant, the offspring populations were tall and dwarf in equal ratio. Work out a cross to show how is it possible.
A heavily bleeding and bruised road accident victim was brought to a nursing home. The doctor immediately gave him an injection to protect him against a deadly disease.
(a) Write what did the doctor inject into the patient's body.
(b) How do you think this injection would protect the patient against the disease?
(c) Name the disease against which this injection was given and the kind of immunity it provides.
Streptococcus: produces streptokinase enzyme. This enzyme is used as a ‘Clot Buster’ that is used to dissolve and removes clots formed in the blood vessels of patients who have had a heart attack.
Monascus: produces statins that act as blood cholesterol lowering agents. They act by competitively inhibiting the enzyme responsible for the synthesis of cholesterol.
Rearrange the following in the current sequences to accomplish an important biotechnological reaction :
(a) In vitro synthesis of region of DNA of interest
(b) Chemically synthesized oligonucleotides
(c) Enzyme DNA-polymerase
(d) Complementary region of DNA
(e) Genomic DNA template
(f) Nucleotides provided
(g) Primers
(h) Thermostable DNA-polymerase (from Thermus aquaticus)
(i) Denaturation of ds-DNA