CBSE
The DNA molecule to which the gene of interest is integrated for cloning is called
Transformer
Vector
Template
Template
The figure below is the diagrammatic representation o the E.Coli vector pBR 322. Which one of the given options correctly identifies its certain components(s)?
Ori- original restriction enzyme
rop-reduced osmotic pressure
Hind III, Eco RI-selectable markers
Hind III, Eco RI-selectable markers
The cutting of DNA at specific locations become possible with the discovery of
Restriction enzymes
Probes
Selectable markers
Selectable markers
DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by
centrifugation
polymerase chain reaction
electrophoresis
electrophoresis
The introduction of t-DNA into plates involves
Infection of the plant by agrobacterium tumefaciens
Altering the pH of the soil, then heat-shocking the plants
Exposing the plates to cold for a brief period
Exposing the plates to cold for a brief period
Which of the following is not a feature of the plasmids?
Circular structure
Transferable
Single stranded
Single stranded
Which of the following is not correctly matched for the organism and its cell wall degrading enzyme?
Bacteria -Lysozyme
Plant cells - Cellulase
Algae - Methylase
Algae - Methylase
Which of the following is a restriction endonuclease?
Protease
DNase I
RNase
RNase
The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of
Non-recombinant bacteria containing β-galactosidease
Insertional inactivation of α - galactosidase in recombinant bacteria
Insertional inactivation of α-galactosidase in recombinant bacteria
Insertional inactivation of α-galactosidase in recombinant bacteria
The Taq polymerase enzyme is obtained from
Thiobacillus ferroxidans
Bacillus subtilis
Pseudomonas subtilis
Pseudomonas subtilis