CBSE
Multiple Choice QuestionsSelect the correct order of processing of PCR.
Extension, primer annealing, denaturation
Denaturation, primer annealing, extension
Denaturation, extension, primer annealing
Primer annealing, denaturation, extension
Assertion: In recombinant DNA technology, human genes are often transferred into bacteria (prokaryotes) or yeasts (eukaryotes).
Reason: Both bacteria and yeasts multiply very fast to form huge populations which express the desired gene.
If both assertion and reason are true and reason is the correct explanation of assertion.
If both assertion and reason are true but reason is not the correct explanation of assertion.
If assertion is true but reason is false.
If both assertion and reason are false.
Plasmid vector in DNA recombinant technology means
a virus that transfers gene to bacteria
extra-chromosomal autonomously replicating circular DNA
sticky end of DNA
any fragment of DNA carrying desirable gene.
An example of gene therapy is
production of injectable hepatitis B vaccine
production of vaccines in food crops like potatoes which can be eaten
introduction of gene for adenosine deaminase in persons suffering from Severe Combined Immuno Deficiency (SCID)
production of test tube babies by artificial insemination and implantation of fertilised eggs
Assertion: Restriction enzymes cut the strand of DNA to produce sticky ends.
Reason: Stickiness of the ends facilitates the action of the enzyme DNA polymerase.
If both assertion and reason are true and reason is the correct explanation of assertion.
If both assertion and reason are true but reason is not the correct explanation of assertion.
If assertion is true but reason is false.
If both assertion and reason are false.
A bacterium divides after every 35 min, if a culture containing 105 cells per mL is grown, then cell concentration per mL after 175 mins will be
175105
125105
48105
32105
Specific radioactive identification of ribosomal RNA can be achieved by using C14 labelled
Guanine
Uracil
thymine
Cytocine
The arrangement of genes on chromosomes is
linear
ovoid
diffused
spiral
Transformation in bacteria was discovered by
Lederberg
Griffith
Avery et. al
Tatum
Which of the following statements is false regarding SDS-polyacrylamide gel electrophoresis?
Proteins are separated by molecular weight
SDS is a detergent which gives charge to protein
Large protein move more slowly through gel
SDS is used to maintain the 3-dimensional structure of protein
