CBSE
Multiple Choice QuestionsWhich one among the following is just a cloning plasmid not an expression plasmid?
pBAD-18-Cam
pBCSK
pUC18
pET
Restriction enzymes:
are endonucleases which cleave DNA at specific sites
make DNA complementary to an existing DNA or RNA
cut or join DNA fragments
are required in vectorless direct gene transfer.
What is the first step in the Southern Blot technique
Denaturation of DNA on the gel for hybridization with specific probe
Production of a group of genetically identical cells
Digestion of DNA by restriction enzyme
Isolation of DNA from a nucleated cell such as the one from the scene of crime
Shorter generation time of E. coli compared to eukaryotes may be explained on the basis of
shape
large surface : volume ratio
presence of cell wall
absence of organelles
Polymerase chain reaction is most useful in
DNA synthesis
DNA amplification
protein synthesis
amino acid synthesis
Restriction enzymes are used to cut
single stranded RNA
double stranded DNA
single stranded DNA
double stranded RNA
Assertion : In recombinant DNA technology, human genes are often transferred into bacteria (prokaryotes) or yeast (eukaryote).
Reason : Both bacteria and yeast multiply very fast to form huge populations which express the desired gene.
If both Assertion and Reason are true and the Reason is the correct explanation of the Assertion
If both Assertion and Reason are true but the Reason is not the correct explanation of the assertion
If Assertion is true but Reason is false.
If both Assertion and Reason are false
Which of the following enzymes is used to join bits of DNA?
Ligase
Primase
Endonuclease
DNA polymerase
cDNA probes are copied from the messenger RNA molecules with the help of
restriction enzymes
reverse transcriptase
DNA polymerase
adenosine deaminase
Molecular scissors, which cut DNA at specific site is
pectinase
polymerase
restriction endonuclease
ligase
