The base sequence in one of the strands of DNA is TAGCATGAT.
(i) Give the base sequence of its complementary strand.
(ii) How are these base pairs held together in a DNA molecule?
(iii) Explain the base complementarity rules. Name the scientist who framed this rule.
(a) Sickle celled anaemia in humans is a result of point mutation. Explain.
(b) Write the genotypes of both the parents who have produced a sickle celled anaemic offspring.
(a) Identify (A) and (B) illustrations in the following:
i.
ii.
(b) Write the term given to (A) and (C) and why?
(c) Expand PCR. Mention its importance in biotechnology.
(a) A represents - Sticky end.
B represents - Foreign DNA insert.
(b) The term used for A is Sticky ends, it is called so because they form hydrogen bonds with their complementary cut counterparts. Their stickiness of the ends facilitates the action of the enzyme DNA ligase.
C are called the palindromic nucleotide sequence. These are named so because the sequence of base pairs reads same on the two strands when orientation of reading is kept the same
(c) PCR stands for Polymerase Chain Reaction. PCR is a technique used to synthesize multiple copies of the gene (or DNA) of interest in vitro using two sets of primers (small chemically synthesized oligonucleotides that are complementary to the regions of DNA) and the enzyme DNA polymerase. It is extensively used in the process of gene manipulation.
The diagram above is that of a typical biogas plant. Explain the seQ uence of events occurring in a biogas plant. Identify a, b and c.
How can crop varieties be made disease resistant to overcome food crisis in India? Explain.
Name one disease resistant variety in India of:
(a) Wheat to leaf and stripe rust
(b) Brassica to white rust
Write the source and the effect on the human body of the following drugs:
(i) Morphine
(ii) Cocaine
(iii) Marijuana
Name the type of interaction seen in each of the following examples:
(i) Ascaris worms living in the intestine of human
(ii) Wasp pollinating fig inflorescence
(iii) Clown fish living among the tentacles of sea-anemone
(iv) Mycorrhizae living on the roots of higher plants
(v) Orchid growing on a branch of a mango tree
(vi) Disappearance of smaller barnacles when Balanus dominated in the Coast of Scotland.
(a) Draw a labelled diagram of the human female reproductive system.
(b) Enumerate the events in the ovary of a human female during:
(i) Follicular phase
(ii) Luteal phase of menstrual cycle