Choose the correct sequence of events in a next-generation sequencing technology-bases whole genome sequencing project.

• DNA extraction → shearing → library preparation → sequencing → assembly → finishing → annotation → submission to Genbank.

• DNA extraction → library preparation → sequencing → assembly → annotation → finishing → submission to Genbank

• DNA extraction → shearing → adapter ligation → library amplification → sequencing → assembly → finishing → annotation → submission to Genbank.

• DNA extraction → adapter ligation → library amplification → shearing → sequencing → finishing → assembly → annotation → submission to Genbank.

142.

An investigator discovers a new receptor for a known ligand and wanted to identify the binding partner of the receptor i.e. its co-receptor. The anti receptor antibody is not available but anti-GFP-antibody is available. Which one of the following strategies is most likely to identify the co-receptor?

• The GFP-receptor fusion protein is expressed in a cell line and analyzed by LC-MS/ MS.

• The GFP-receptor fusion protein is expressed in a cell line and the cells positive for GFP were sorted out, lysed and run on a polyacrylamide gel.

• The GFP-receptor fusion protein is coated on ELISA plate, followed by ELISA with an anti-GFP antibody.

• The receptor is cloned as a fucion protein of GFP and expressed in stimulated cells. The immuno-precipitated complex obtained by anti-GFP antibody was analyzed by LC-MS/ MS.

The frequency distribution of tree heights in two forest areas with different annual rainfall are given

Which of the following statistical analysis will you choose to test whether rainfall has an effect on tree heights?

• t test for comparison of means.

• A non-parametric comparison of the two groups.

• Correlation analysis of rainfall and mean tree heights.

• Regression of tree heights on rainfall.

Two species of plants were sampled in 32 quadrants in a forest. The mean and variance for the occurrence of species 1 were 16.2 and 48 and species 2 were 3.6 and 3.2 respectively. Which of the following statements about the distribution of the two species in these quadrats is supported by these findings?

• Both species are distributed randomly.

• Species 1 is distributed randomly and species 2 is clustered.

• Species 1 is clustered and species 2 is distributed randomly.

• Both species are clustered.

Poly-L-lysine exits in pure α-helix, β-sheet and random coiled conformations depending upon the solvent conditions. The values of mean residue ellipticity at 220 nm ([θ]₂₂₀) are -35,700, -13,800 and +3,900 deg cm² dmol^-1 for α-helix, β-sheet and random coil conformations of this polypeptide, respectively. The polypeptide exists in α-helix conformation at pH 10.8 and 25°C. Addition of urea leads to a two state transition between α-helix and random coil conformation. It has been observed that [θ]₂₂₂ of the polypeptide is -14800 deg cm²dmol^-1 in the presence of 6M urea. The percentage of the polypeptide in α-helix conformation is:

• 37

• 41

• 47

• 50