Polymerase Chain Reaction or PCR is used to amplify the gene of interest. In this method -:
Primers are designed according to the sequence of the gene of interest.
Two sets of primers (chemically synthesized oligonucleotide stretches) that are complementary to the gene of interest, DNA polymerase enzyme, and deoxynucleotides are added to the PCR mixture.
PCR consists of 3 steps:
i. Denaturation The ds DNA is denatured by providing high temperature. Taq DNA polymerase isolated from the thermophilic bacteria, Thermus aquaticus (Taq), which does not degrade at such high temperatures is used.
