141.

Insertion lambda vectors are used to create cDNA librabries, in some insertion vectors, the site of insertion is within the cI gene. The recombinants which are cI‐ can then be screened by plating on E‐coli hfI. The gene hfI encodes a protease that control lytic‐lysogeny decision through which mechanism?

• HfI protease degrade cI, thereby promoting lytic pathway

• HfI protease degrade cIII and so cI cannot interact with cII lysogenic pathway is thus preferred

• HfI protease degrades cII and therefore cI synthesis cannot be established, thus lytic pathway is preferred

• HfI rotease degrade INT, the protein involved in phage integration and the lytic pathway is initiated

Industrial products in which bacteria are employed for production are shown in the following table:

I. List of product	II. Microorganism
A. 2,3- Butane diol	i) Leuconostoc
B. Dextran	ii) Brevibacterium
C. Glutamic acid	iii) Bacillus polymxa
D. Cobalamine	iv) Propionibacterium

The correct combinations are:

• A – iii; B – i; C – ii; D – iv

• A – i; B – ii; C – iii; D – iv

• A – iii; B – ii; C – iv; D – i

• A – ii; B – iii; C – iv; D – i

Show the graph below are the fitness costs and benefits of four alternatives behavioral phenotypes (P, Q, R, S). Given sufficient evolutionary time, which phenotype(s) is likely to evolve as an adaptation?

• Q and R

• Q only

• P, Q and R

• S only

The following geological areas mark the advent of important events in the history of earth origin of terrestrial plants, origin of mammals and breakup of the
lad masses Pangea:
(a). Early Cambrian
(b). Late Devonian
(c). Late cretaceous
(d). Late Jurassic
Identify the correct match of the events with the geological era:

• Origin of terrestrial plants (c); origin of mammals (d); break up of Pangaea (a)

• Origin of terrestrial plants (b); origin of mammals (d); break up of Pangaea (c)

• Origin of terrestrial plants (d); origin of mammals (c); break up of Pangaea (b)

• Origin of terrestrial plants (b); origin of mammals (c); break up of Pangaea (d)

A reference material is required to be prepared with 4 ppm calcium. The amount of CaCO₃ (molecular weight = 100) required to prepare 1000 g of such a reference material is

• 10 µg

• 4 µg      

• 4 mg

• 10 mg